特殊螺纹接头双主密封结构密封性能分析

针对油气井日益苛刻的环境,基于特殊螺纹密封机制,提出一种由锥面对锥面和柱面对球面组合的双主密封螺纹接头结构。采用有限元法对比分析双主密封、锥面对锥面密封、柱面对球面密封3种主密封结构在上扣、拉伸、压缩、拉伸和内压、拉伸和内外压5种工况下,密封面接触应力、接触长度的变化情况,得到3种主密封结构的密封能力随载荷的变化规律。分析结果表明,与单主密封接头相比,该双主密封接头在不同载荷下锥面和柱面上的接触应力分布更均匀,接触长度更长且接触应力峰值更小。全尺寸试验结果表明,该双主密封接头的密封性能达到了使用要求。     

Targeted editing of intronic-splicing silencer enhancement of SMN2 Exon 7 inclusion by CRISPR/Case 9

Spinal muscular atrophy (SMA) is an autosomal recessive hereditary neuromuscular disease. Exon 7 and 8 of survival of motor neuron 1 (SMN1) gene or only exon 7 homology deletion leads to the failure to produce a full-length SMN gene. The copy number of SMN2 gene with high homology of SMN1 affects the degree of disease and was the target gene for targeting therapy, in which splicing silencer in intron 7 was the key to suppress the inclusion of exon 7. In this study, we projected to use CRISPR/Case 9 for the targeted editing of intronic-splicing silencer (ISS) sequence to promote the inclusion of SMN2 exon 7 and increase the production of SMN2 full-length (FL) gene expression. It happens that there was a protospacer adjacent motif (PAM) at one end of the ISS sequence according to the design of sgRNA. The recombinant vector of sgRNA HSMN2 CRISPR/Case 9 was constructed and transfected into HEK293 cells. Sequencing results showed that the ISS sequence could be edited accurately and targeting in the predicted direction, in which deleting small fragments, inserting small amounts and mutation. Quantitative analysis of RT-PCR products by restriction enzyme of DdeI digestion showed that the FL of SMN2 increased by 8% (P < 0.05). In the primary cultured chondrocytes of SMA mice, in which sgRNA HSMN2 CRISPR/Case9 recombinant vector transfection could increase the SMN2 FL gene by 23% (P < 0.05) and significantly improve SMN protein levels (P < 0.05). CRISPR/Case 9 is an effective tool for gene editing and therapy of hereditary diseases, but it is rarely reported in the treatment of SMA diseases. This study shows that CRISPR/Case 9 was first used for the precision target of ISS sequence editing, which can effectively promote the production of SMN2 FL gene expressions, in which there was an important clinical reference value.     

Dynamic response of a beam to the train of moving forces driven by an Erlang renewal process

Dynamic response of a beam to a random train of moving forces moving with the same velocity is considered. Unlike a widely used Poisson process model, a more adequate Erlang renewal process is used as a process driving the train of forces. Normal-mode approach is used to convert the problem into that of a renewal driven train of general pulses. Consequently the modal responses are the filtered renewal processes and are expressed as integrals with respect to the response to a single pulse (passage of a force) and to the increments of the counting renewal process. The expressions for the mean values and cross-correlations of modal responses are obtained as single and double integrals, respectively. The results are presented in terms of the renewal density of the underlying Erlang counting process. Mean value and variance of the mid-span deflection of the beam are determined by numerical evaluation of the pertinent integrals. Numerical analysis is carried out for the Erlang processes with integer parameter $k=2$, $k=3$ and 4 and, for comparison, also for a Poisson process. Different traffic conditions such as the velocity and the mean arrival rate of vehicles are taken into account.     

基于工程状态标定的诊断载荷试验识别方法及应用

针对在役公路梁式桥梁由于材料性能退化及外部环境等因素共同作用而导致承载力不足、耐久性降低等问题,开展旧桥力学性能的快速诊断试验研究。通过诊断性载荷试验的理论分析和有限元模型标定流程研究,快速、高效地完成在役公路桥梁的承载力性能测量和评估,从而为在役公路桥梁结构的养护管理提供可靠的技术支持。     

温州某公轨共建高架车站结构设计

为了解决城市廊道资源的紧张和公共交通发展需求的矛盾,出现了轨道交通和快速路的共建型式,即公轨共建的复合型交通模式,以温州市域铁路S2线一期工程的人民路站为例,研究公轨共建高架车站的结构设计要点。首先,为降低浅层气体对桩基的承载力及沉降影响,对遇到局部浅层气体发育的基础设计提供了合理的解决方案。然后针对公轨共建一体化车站分别进行了多遇和罕遇地震作用分析,研究表明构件及整体指标满足规范要求。最后针对工程的一些关键技术,如快速路桥面体系的选型、超长结构温度应力的控制措施、轨道梁挠度及应力的控制、快速路桥面振动对车站的影响等进行了分析,并给出了具体的解决方案和指导意见。     

曲线双工字钢组合梁桥横梁受力分析研究

为了研究主梁腹板纵桥向翘曲变形对横梁内力的影响,在现有横梁框架模型基础上,根据符拉索夫薄壁结构理论得到集中荷载作用下简支曲线双工字钢组合梁桥翘曲变形、结构扭转角等物理参数沿跨径的分布规律。利用主梁腹板与横梁的变形协调关系分析横梁的纵桥向变形,得到横梁内力组成以及截面正应力分布规律并进行有限元分析验证。结果表明:基于符拉索夫薄壁结构理论分析得到的横梁截面正应力分布规律与有限元计算得到的规律基本一致; 靠近加载点的横梁正应力以“竖向弯曲效应”为主导,靠近支座截面的横梁“腹板变形不一致效应”大于加载点截面附近横梁; 加载点截面两侧腹板翘曲变形方向相反,导致横梁的截面正应力分布规律相反; 横梁竖向弯曲变形产生的应力与主梁弯矩分布规律类似,纵桥向弯曲变形产生的应力与扭率分布规律类似。     

横向行驶简支桥板最大内力计算方法探讨

同跨度的横向行驶的简支桥板与通常纵向行驶的简支桥板，在相同设计标准条件下，由于荷载分布情况不同，致使最不利截面和最大内力计算不尽相同，针对横向行驶的简支桥板最大内力计算十分烦琐的特点，推导出较为简便的计算公式，以供参考。     

全数字化地形图测量数据检测精度分析

针对数字化产品具有数据量大、用途多、技术方法多样等特点,根据用户对全数字化地形图的内容和精度要求较高的情况,提出在全数字地形测量中应加强质量控制、采取分阶段参照监理程序,对成果数据进行监控检核度分析,以保证最终成果质量能满足精度要求的技术方案。     

布曲河特大桥钻孔灌注桩施工工艺

在高原多年冻土地区，气候恶劣，生态环境脆弱，钻孔灌注桩施工中又不可避免地会遇到土层处于不同的冻结状态，施工中必须考虑并设法预防和消除由于冻土的变形、强度弱化及冻胀、融沉所引起的各种病害，因此钻孔灌注桩施工在质量、安全和环保等方面难度很大。介绍了布曲河特大桥钻孔灌注桩施工工艺，该工艺明显不同于内地通常的钻孔灌注桩施工工艺，在高原多年冻土地区是行之有效的，对高原多年冻土区钻孔灌注桩施工具有重要的参考意义。